정보공개
Effects of Brown Rice Extract Treated with Lactobacillus sakei Wikim001 on Osteoblast Differentiatio
- 작성일2017.05.15 00:00
- 분류
- 조회수1,873
- 링크https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4287330/
- 저널명Preventive Nutrition and Food Science
- 저자강미란,송정희,김태운,박성희,이종희,박해웅
- 구분
- 유형
제목
Effects of Brown Rice Extract Treated with Lactobacillus sakei Wikim001 on Osteoblast Differentiation and Osteoclast Formatio
요약
Phytic acid (myo-inositol hexakisphosphate) or phytate is considered an anti-nutrient due to the formation of precipitated complexes that strongly reduces the absorption of essential dietary minerals. In this study, brown rice with reduced phytate was made by inoculation with Lactobacillus sakei Wikim001 having high phytase activity. The effects of brown rice extract treated with L. sakei Wikim001 (BR-WK) on osteoblast differentiation and osteoclast formation were investigated. The proliferation of SaOS-2 cells was measured by the MTT assay. Treatment with BR-WK increased cell proliferation by 136% at a concentration of 100 ?g/mL. The Alkaline phosphate activity in SaOS-2 cells was 129% higher when BR-WK was processed at a concentration of 100 ?g/mL. The proliferation of bone marrow macrophages decreased by nearly 60% in response to treatment with BR-WK. In addition, BR-WK reduced the number of tartrate-resistant acid phosphatase-positive (TRAP+) multinucleated cells from bone marrow macrophages. These results indicate that BR-WK stimulates bone formation through its positive action on osteoblast differentiation and function and furthermore, decreases osteoclast differentiation.
콘텐츠 정보책임자
- 담당부서 사업예산실
- 담당자 고재덕
- 연락처 062-610-1708